Description
The NCM460D cell line is an epithelial cell line derived from the normal colon of a 68-year-old Hispanic male (*Moyer et al., 1996). The line was selected for in vitro growth. It was not infected or transfected with any exogenous genetic information. The cells express cytokeratins, villin, and other colonic epithelial cell antigens associated with other cell types. Some of the cells are positive for mucin synthesis. They do not grow in soft agar and are non-tumorigenic. They have fastidious growth requirements and must be maintained in INCELL’s enriched M3:10™ medium for long-term in vitro culture with maintenance of an appropriate phenotype.
